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High-throughput Sequencing Specification

Specification of Illumna Platform

Platform Run Cycle Base per Reads (bp) Read per Lane (M) Lane per Cell Cell per Run Run Reagent Run Time (h) Q30 (%)
iSeq100 SE36 36 4 1 1 - 9 85
iSeq100 SE50 50 4 1 1 - 9 85
iSeq100 SE50 75 4 1 1 - 10 80
iSeq100 PE50 150 4 1 1 - 13 80
iSeq100 PE150 300 4 1 1 - 17.5 80
MiniSeq SE75 75 22–25 1 1 High 7 85
MiniSeq PE75 150 22–25 1 1 High 13 85
MiniSeq PE150 600 22–25 1 1 High 24 80
MiniSeq PE150 600 7-8 1 1 Mid 17 80
MiSeq PE75 150 22–25 1 1 v3 21 85
MiSeq PE300 600 22–25 1 1 v3 56 70
NextSeq550 SE75 75 400 1 1 High 11 80
NextSeq550 PE75 150 400 1 1 High 18 80
NextSeq550 PE150 300 400 1 1 High 29 75
NextSeq550 PE75 150 130 1 1 Mid 15 80
NextSeq550 PE150 300 130 1 1 Mid 26 75
HiSeq2000 PE150 300
HiSeq2500 PE150 300
HiSeq3000 PE150 300
HiSeq4000 PE150 300
HiSeqX PE150 300 325-375 8 1/2 - 70 75
NovaSeq6000 PE50 100 325-400 2 1/2 SP 13 85
NovaSeq6000 PE150 300 325-400 2 1/2 SP 25 75
NovaSeq6000 PE250 500 325-400 2 1/2 SP 38 75
NovaSeq6000 PE50 100 650-800 2 1/2 S1 13 85
NovaSeq6000 PE100 200 650-800 2 1/2 S1 19 80
NovaSeq6000 PE150 300 650-800 2 1/2 S1 25 75
NovaSeq6000 PE50 100 1650-2050 2 1/2 S2 16 85
NovaSeq6000 PE100 200 1650-2050 2 1/2 S2 25 80
NovaSeq6000 PE150 300 1650-2050 2 1/2 S2 36 75
NovaSeq6000 PE100 200 2000-2500 4 1/2 S4 36 80
NovaSeq6000 PE150 300 2000-2500 4 1/2 S4 44 75

note:

  • paired end reads are regarded as one
  • there are unreggle 4 lanes on MiSeq chip, thus treated as one
  • 1/2 means single flow cell and dual flow cell are both supported

Specification of PacBio SMRT Platform


Last modified on 2020-01-30