High-throughput Sequencing Specification
Specification of Illumna Platform
| Platform | Run Cycle | Base per Reads (bp) | Read per Lane (M) | Lane per Cell | Cell per Run | Run Reagent | Run Time (h) | Q30 (%) |
|---|---|---|---|---|---|---|---|---|
| iSeq100 | SE36 | 36 | 4 | 1 | 1 | - | 9 | 85 |
| iSeq100 | SE50 | 50 | 4 | 1 | 1 | - | 9 | 85 |
| iSeq100 | SE50 | 75 | 4 | 1 | 1 | - | 10 | 80 |
| iSeq100 | PE50 | 150 | 4 | 1 | 1 | - | 13 | 80 |
| iSeq100 | PE150 | 300 | 4 | 1 | 1 | - | 17.5 | 80 |
| MiniSeq | SE75 | 75 | 22–25 | 1 | 1 | High | 7 | 85 |
| MiniSeq | PE75 | 150 | 22–25 | 1 | 1 | High | 13 | 85 |
| MiniSeq | PE150 | 600 | 22–25 | 1 | 1 | High | 24 | 80 |
| MiniSeq | PE150 | 600 | 7-8 | 1 | 1 | Mid | 17 | 80 |
| MiSeq | PE75 | 150 | 22–25 | 1 | 1 | v3 | 21 | 85 |
| MiSeq | PE300 | 600 | 22–25 | 1 | 1 | v3 | 56 | 70 |
| NextSeq550 | SE75 | 75 | 400 | 1 | 1 | High | 11 | 80 |
| NextSeq550 | PE75 | 150 | 400 | 1 | 1 | High | 18 | 80 |
| NextSeq550 | PE150 | 300 | 400 | 1 | 1 | High | 29 | 75 |
| NextSeq550 | PE75 | 150 | 130 | 1 | 1 | Mid | 15 | 80 |
| NextSeq550 | PE150 | 300 | 130 | 1 | 1 | Mid | 26 | 75 |
| HiSeq2000 | PE150 | 300 | ||||||
| HiSeq2500 | PE150 | 300 | ||||||
| HiSeq3000 | PE150 | 300 | ||||||
| HiSeq4000 | PE150 | 300 | ||||||
| HiSeqX | PE150 | 300 | 325-375 | 8 | 1/2 | - | 70 | 75 |
| NovaSeq6000 | PE50 | 100 | 325-400 | 2 | 1/2 | SP | 13 | 85 |
| NovaSeq6000 | PE150 | 300 | 325-400 | 2 | 1/2 | SP | 25 | 75 |
| NovaSeq6000 | PE250 | 500 | 325-400 | 2 | 1/2 | SP | 38 | 75 |
| NovaSeq6000 | PE50 | 100 | 650-800 | 2 | 1/2 | S1 | 13 | 85 |
| NovaSeq6000 | PE100 | 200 | 650-800 | 2 | 1/2 | S1 | 19 | 80 |
| NovaSeq6000 | PE150 | 300 | 650-800 | 2 | 1/2 | S1 | 25 | 75 |
| NovaSeq6000 | PE50 | 100 | 1650-2050 | 2 | 1/2 | S2 | 16 | 85 |
| NovaSeq6000 | PE100 | 200 | 1650-2050 | 2 | 1/2 | S2 | 25 | 80 |
| NovaSeq6000 | PE150 | 300 | 1650-2050 | 2 | 1/2 | S2 | 36 | 75 |
| NovaSeq6000 | PE100 | 200 | 2000-2500 | 4 | 1/2 | S4 | 36 | 80 |
| NovaSeq6000 | PE150 | 300 | 2000-2500 | 4 | 1/2 | S4 | 44 | 75 |
note:
- paired end reads are regarded as one
- there are unreggle 4 lanes on MiSeq chip, thus treated as one
- 1/2 means single flow cell and dual flow cell are both supported
Specification of PacBio SMRT Platform
Last modified on 2023-11-01